Aminobenzofuran-Fused Rhodamine Dyes with Deep-Red to Near-Infrared Emission for Biological Applications
氨基呋喃罗丹明染料在深红和近红外光谱发射在生物领域的应用
通过3-氨基苯并呋喃酮衍生物可以高效地缩合合成氨基呋喃罗丹明化合物染料（AFR dyes），该化合物展示了出色的生物性能，包括在深红和近红外的光谱发射，大的斯托克斯位移
良好的对光和酸碱的稳定性！
Aminobenzofuran-fused rhodamine dyes (AFR dyes) containing an amino group were constructed by an efficient condensation based on 3-coumaranone derivatives. AFR dyes exhibited significantly improved properties, including deep-red and near-infrared emissions, a large Stokes shift, good photostability, and wide pH stability. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium assay experiments show that these AFR dyes are biocompatible for their low cytotoxicity to both A549 and HeLa cells. Cell imaging data reveal that AFR1, AFR1E, and AFR2 are mainly located in the mitochondria, while AFR3 is a lysosome tracker. As far as we know, NIR AFR3 is the longest fluorescent rhodamine derivative containing the amino group. These amino group-containing AFR dyes hold great potential in fluorogenic detection, biomolecule labeling, and cell imaging.
Guangle Niu †‡, Weimin Liu *†, Jiasheng Wu †, Bingjiang Zhou †‡, Jianhong Chen †‡, Hongyan Zhang †, Jiechao Ge †, Ying Wang †, Haitao Xu †, and Pengfei Wang †
† Key Laboratory of Photochemical Conversion and Optoelectronic Materials and CityU-CAS Joint Laboratory of Functional Materials and Devices, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
‡ University of Chinese Academy of Sciences, Beijing 100049, China
J. Org. Chem
